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A student builds a plasmid that carries a gene for… | StemPy Lab
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A student builds a plasmid that carries a gene for kanamycin resistance and a single EcoRI site, but no origin of replication. What is the most likely result in a bacterial host?
Biology
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High School (11–12)
Biotechnology › Biotechnology: Principles and Processes
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A student builds a plasmid that carries a gene for kanamycin resistance and a single EcoRI site, but no origin of replication. What is the most likely result in a bacterial host?
The plasmid cannot copy itself, so the number of plasmids per cell falls as the cells divide
The plasmid copies itself in the usual way, but the cells cannot be selected
The plasmid copies itself, but only in the cells that already have kanamycin resistance
The plasmid cuts itself at the EcoRI site and is lost
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← After a restriction enzyme has cut a vector and a gene, the two are mixed and the sticky ends pair. What is the job of DNA ligase?
One piece of DNA was cut with HindIII, and a vector was cut with EcoRI. The student mixes them and adds DNA ligase. What is the most likely result? →
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Biotechnology › Biotechnology: Principles and Processes
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