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One piece of DNA was cut with HindIII, and a vector was… | StemPy Lab
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One piece of DNA was cut with HindIII, and a vector was cut with EcoRI. The student mixes them and adds DNA ligase. What is the most likely result?
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High School (11–12)
Biotechnology › Biotechnology: Principles and Processes
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One piece of DNA was cut with HindIII, and a vector was cut with EcoRI. The student mixes them and adds DNA ligase. What is the most likely result?
Few recombinants, because the sticky ends AGCT and AATT do not pair
Many recombinants, because both enzymes cut DNA
Many recombinants, because ligase can join any two ends
No ligation at all, because ligase needs a blunt end
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← A student builds a plasmid that carries a gene for kanamycin resistance and a single EcoRI site, but no origin of replication. What is the most likely result in a bacterial host?
Compared with an enzyme that recognises a 4-base sequence, an enzyme that recognises an 8-base sequence is expected, in a long random DNA, to cut →
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Biotechnology › Biotechnology: Principles and Processes
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