Five statements about the chapter are given…
Contributed by StemPy LabTry it on paper first. A hint, the key idea and the worked solution are here when you need them.
Five statements about the chapter are given. Two are wrong. Find them and correct them.
- A white colony on a medium with a chromogenic substrate has a plasmid without an insert.
- DNA is cut out of the gel after electrophoresis, and the step is called elution.
- A sparged bioreactor has no stirrer.
- The overall yield of three steps is the product of the three yields.
- Taq polymerase comes from Thermus aquaticus.
Need a reminder? The key ideaShow
Downstream processing is separation, purification, formulation with preservatives and quality control. A drug also goes through clinical trials. The overall yield is the product of the yields of the steps.
The worked solution
Have you tried it?
Work it out on paper first. It is fine to get stuck or make a mistake: that is how we learn. When you are ready, go through the solution one step at a time, and check each step against your own.
Given. Five statements.
Plan. Test each one.
Step 1. Statement 1 is wrong. A white colony has an insert in lacZ. A plasmid without an insert gives a blue colony.
Step 2. Statement 2 is right. The band is cut out and the DNA is eluted.
Step 3. Statement 3 is wrong. A sparged stirred-tank reactor has a stirrer and also bubbles sterile air.
Step 4. Statement 4 is right. The shares multiply.
Step 5. Statement 5 is right.
Answer: Statements 1 and 3 are wrong. Correct: a white colony is a recombinant; a sparged reactor has a stirrer and bubbles of air.
Check. Three of the five statements are right.
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