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After a restriction enzyme has cut a vector and a gene… | StemPy Lab
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After a restriction enzyme has cut a vector and a gene, the two are mixed and the sticky ends pair. What is the job of DNA ligase?
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Biotechnology › Biotechnology: Principles and Processes
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After a restriction enzyme has cut a vector and a gene, the two are mixed and the sticky ends pair. What is the job of DNA ligase?
It joins the sugar-phosphate backbone of the two pieces, so that the joint is permanent
It makes the sticky ends longer so that they pair more strongly
It cuts the strand again at the recognition sequence to correct the joint
It adds methyl groups to the DNA so that the joint is protected
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← A circular plasmid has six sites for a restriction enzyme, and the enzyme cuts at all of them. How many fragments are formed?
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