A culture of *Escherichia coli* is diluted by a factor of 10⁶ and 0.1 mL of the dilution is spread on an agar plate, which gives 85 colonies. The cells in the culture double every 30 min and have been growing exponentially for the last 120 min at least. What was the number of viable cells per mL in the culture 120 min earlier?

BiologyHardHigh School (11–12)Heredity and Evolution › Evolution Olympiad

A culture of Escherichia coli is diluted by a factor of $10^{6}$ and $0.1\ \text{mL}$ of the dilution is spread on an agar plate, which gives $85$ colonies. The cells in the culture double every $30\ \text{min}$ and have been growing exponentially for the last $120\ \text{min}$ at least. What was the number of viable cells per $\text{mL}$ in the culture $120\ \text{min}$ earlier?

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