A PCR copies a product of 2400 bp. Each cycle has denaturation for 30 s, annealing for 30 s and…
Contributed by StemPy LabTry it on paper first. A hint, the key idea and the worked solution are here when you need them.
A PCR copies a product of bp. Each cycle has denaturation for s, annealing for s and extension at one minute for each kilobase. The run has cycles.
- How long is the extension step, in seconds?
- How long is one cycle?
- How long is the whole run, in minutes?
Need a reminder? The key ideaShow
A primer has 18 to 25 bases, 40 to 60 per cent G and C, and a G or C at the 3 prime end. , and the annealing temperature is about 5 degrees Celsius below it. Extension takes about 1 minute for each kilobase. The number of bases between the start codon and the insert must be a multiple of 3.
The worked solution
Have you tried it?
Work it out on paper first. It is fine to get stuck or make a mistake: that is how we learn. When you are ready, go through the solution one step at a time, and check each step against your own.
Given. bp; s and s; cycles.
Plan. Add the three steps of a cycle, and multiply by the cycles.
Step 1. (a) s.
Step 2. (b) s.
Step 3. (c) s, which is min.
Answer: s; s; min.
Check. The extension is the longest step of the cycle, as it should be for a product of this size.
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