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What an enzyme is, the active site and the lowering of activation energy. Then how temperature, pH, substrate and inhibitors change the rate, the six classes of enzyme, and cofactors, coenzymes and prosthetic groups.
Beyond the textbook: how proteins fold and why shape means function, and Michaelis-Menten kinetics with the Lineweaver-Burk plot. Then competitive and non-competitive inhibition by numbers, and how pathways are controlled by feedback, with a full timed paper.