From Gene to Product: Bioreactors and Downstream Processing

Name: From Gene to Product: Bioreactors and Downstream Processing
Author: StemPy Lab

A recombinant cell on a plate is only the beginning. To sell a medicine, you need litres and litres of it. You see how the foreign gene is expressed and how a culture grows, how a bioreactor keeps thousands of litres of cells alive, and how the product is separated, purified and tested. Then you put the whole chapter in order and learn its usual traps. It serves the Indian syllabus (NCERT, NEET) and the matching topic of A-Level, IB and AP.

3 lessons, about 1 h 15 min3 simulations11 problems3 practice questionsPython in every lesson

Created by StemPy Lab

Course outline (3 lessons)

Lesson 1about 25 min
Expressing the foreign gene and growing the culture
A cloned gene is useful only when the host makes its protein. A protein made by a gene of another species is a recombinant protein. A small culture in a flask gives little product, so the culture is grown on a large scale. A batch culture grows through a lag phase, a log phase and a stationary phase.
1 simulation1 practice question1 Python program3 problems1 Python lab
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Lesson 2about 25 min
Bioreactors: stirred-tank and sparged-tank
A bioreactor is a vessel in which cells turn raw materials into a product under controlled conditions. NCERT describes two kinds. The stirred-tank reactor has a stirrer that mixes the culture and carries oxygen through it. In the sparged stirred-tank reactor, sterile air is also bubbled in. A bioreactor has systems to control temperature, pH, foam and oxygen, and ports for taking samples.
1 simulation1 practice question1 Python program4 problems
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Lesson 3about 25 min
Downstream processing, and the chapter in order
After the culture has made the product, the product must be separated, purified, formulated with preservatives and tested, and a medicine goes through clinical trials. These are the steps of downstream processing, and each one loses a part of the product. The lesson ends with the steps of the whole technology in order, and with the usual traps.
1 simulation1 practice question1 Python program4 problems1 Python lab1 question set1 note
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Biotechnology: Principles and Processes: Chapter Test

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From Gene to Product: Bioreactors and Downstream Processing

Course outline (3 lessons)

Expressing the foreign gene and growing the culture

Bioreactors: stirred-tank and sparged-tank

Downstream processing, and the chapter in order

Test Your Learning

Your teacher

Discussion (0)