The culture in a bioreactor holds 500 g of a protein…
Contributed by StemPy LabTry it on paper first. A hint, the key idea and the worked solution are here when you need them.
The culture in a bioreactor holds g of a protein. Three steps of downstream processing keep per cent (separation), per cent (purification) and per cent (formulation) of the product.
- How many grams are left after the purification?
- How many grams of the finished product are there?
- What is the overall yield, as a per cent?
Need a reminder? The key ideaShow
Downstream processing is separation, purification, formulation with preservatives and quality control. A drug also goes through clinical trials. The overall yield is the product of the yields of the steps.
The worked solution
Have you tried it?
Work it out on paper first. It is fine to get stuck or make a mistake: that is how we learn. When you are ready, go through the solution one step at a time, and check each step against your own.
Given. g. Shares , and .
Plan. Multiply the amount by each share in turn.
Step 1. After separation: g.
Step 2. (a) After purification: g.
Step 3. (b) After formulation: g.
Step 4. (c) per cent.
Answer: g; g; per cent.
Check. , the same as the overall yield.
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