An enzyme was tested at five substrate concentrations…
Contributed by StemPy LabTry it on paper first. A hint, the key idea and the worked solution are here when you need them.
An enzyme was tested at five substrate concentrations. The table gives the rates.
| [S] (mmol/L) | 1 | 3 | 6 | 12 | 27 |
|---|---|---|---|---|---|
| v (µmol/min) | 22.5 | 45 | 60 | 72 | 81 |
- Fit the double reciprocal line and find .
- Find .
- Predict the rate at 15 mmol/L.
Need a reminder? The key ideaShow
. At the rate is half of . . On the double reciprocal plot the y-intercept is , the x-intercept is and the slope is .
The worked solution
Have you tried it?
Work it out on paper first. It is fine to get stuck or make a mistake: that is how we learn. When you are ready, go through the solution one step at a time, and check each step against your own.
Step 1. The line. The reciprocals run 1, 0.333, 0.167, 0.0833 and 0.0370, and runs 0.0444, 0.0222, 0.0167, 0.0139 and 0.0123. A straight line through them has a y-intercept of 0.0111 and a slope of 0.0333.
Step 2. The constants. µmol/min. mmol/L.
Step 3. The prediction. µmol/min.
Answer: µmol/min, mmol/L, and 75 µmol/min at 15 mmol/L.
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