Try it on paper first. A hint, the key idea and the worked solution are here when you need them.
A solution holds an enzyme at 2.5 nmol/L. When the enzyme is saturated, the solution makes 150 µmol of product in each litre every minute. The enzyme has mmol/L.
. At the rate is half of . . On the double reciprocal plot the y-intercept is , the x-intercept is and the slope is .
Have you tried it?
Work it out on paper first. It is fine to get stuck or make a mistake: that is how we learn. When you are ready, go through the solution one step at a time, and check each step against your own.
Step 1. . µmol/(L s).
Step 2. . The enzyme is 2.5 nmol/L, which is 0.0025 µmol/L. So per second.
Step 3. The efficiency. mmol/L mol/L. So L/(mol s).
Answer: 2.5 µmol/(L s); 1000 per second; L/(mol s) (2.0e6).
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