Try it on paper first. A hint, the key idea and the worked solution are here when you need them.
A test cross of PQR over pqr with pqr over pqr gives offspring in eight classes. The letters are written in the order P, Q, R.
The gene order is not known.
In a three-point test cross, the two commonest classes are parental and the two rarest are double crossovers. The gene that switches in a double crossover is in the middle. A recombination frequency counts the single and the double crossovers of the interval. The coefficient of coincidence is the observed divided by the expected double crossovers.
Have you tried it?
Work it out on paper first. It is fine to get stuck or make a mistake: that is how we learn. When you are ready, go through the solution one step at a time, and check each step against your own.
Given. Parental: PQR and pqr. Rarest: PqR and pQr.
Plan. Find the gene that differs in the double crossovers. Then count the recombinants of each interval, with the double crossovers included.
Step 1. (a) PqR differs from PQR in the gene Q only. So Q is in the middle, and the order is P, Q, R.
Step 2. (b) The classes Pqr and pQR are single crossovers between P and Q (). Add the double crossovers (). Total of , so map units.
Step 3. (c) PQr and pqR are single crossovers between Q and R (). Add the double crossovers (). Total of , so map units.
Step 4. (d) The map distance is map units. By direct count, P and R show only recombinants, because the double crossovers cancel.
Answer: (a) Q; (b) map units; (c) map units; (d) map units.
Check. .
Watch out. The order in which the letters are written is not the order on the chromosome.
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