Try it on paper first. A hint, the key idea and the worked solution are here when you need them.
A plant of the genotype DEF over def is test crossed with def over def. Of offspring, the eight classes are counted (the genes are listed in the order D, E, F as written).
In a three-point test cross, the two commonest classes are parental and the two rarest are double crossovers. The gene that switches in a double crossover is in the middle. A recombination frequency counts the single and the double crossovers of the interval. The coefficient of coincidence is the observed divided by the expected double crossovers.
Have you tried it?
Work it out on paper first. It is fine to get stuck or make a mistake: that is how we learn. When you are ready, go through the solution one step at a time, and check each step against your own.
Given. The parental classes are DEF and def ( each). The rarest classes are DeF and dEf ( each).
Plan. Find the double crossovers. Then count the recombinants for each interval.
Step 1. (a) offspring. The gene E switches in them, so E is in the middle, and the order is D, E, F.
Step 2. (b) Between D and E: the single crossovers dEF and Def () and the double crossovers (). Total of , which is map units.
Step 3. (c) Between E and F: the single crossovers DEf and deF () and the double crossovers (). Total of , which is map units.
Step 4. (d) Expected double crossovers: . Observed: . The coefficient of coincidence is .
Answer: offspring; map units; map units; .
Check. .
Watch out. The double crossovers count in both intervals. If you leave them out, the distances come out too small.
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