Try it on paper first. A hint, the key idea and the worked solution are here when you need them.
A straight DNA is base pairs long. Enzyme A cuts it at bp and at bp, measured from the left end. Enzyme B cuts it at bp and at bp. The two enzymes do not cut at the same place.
Have you tried it?
Work it out on paper first. It is fine to get stuck or make a mistake: that is how we learn. When you are ready, go through the solution one step at a time, and check each step against your own.
Given. Length bp. A: and . B: and .
Plan. Put the cuts in order, with and at the two ends. The gaps are the fragment sizes.
Step 1. (a) Enzyme A: , , and . That is fragments, and the largest is bp.
Step 2. (b) Enzyme B: , and . That is fragments, and the largest is bp.
Step 3. (c) The cuts in order: , , , . Fragments: , , , and bp. That is fragments. The smallest is bp.
Answer: A: fragments, bp largest. B: fragments, bp largest. Both: fragments, bp smallest.
Check. bp. The pieces add up to the whole.
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A restriction endonuclease cuts both strands inside its palindromic recognition sequence. A site of bases turns up by chance about once in bases. A straight DNA with sites gives fragments. A circular DNA with sites gives fragments.
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