A culture is in its log phase from the start…
Contributed by StemPy LabTry it on paper first. A hint, the key idea and the worked solution are here when you need them.
A culture is in its log phase from the start. It holds cells in every millilitre, and it doubles every minutes. The workers want at least cells in every millilitre before they move the culture to a larger vessel.
- How many doublings are needed?
- How long must they wait, in minutes?
Need a reminder? The key ideaShow
A batch culture has a lag phase, a log phase, a stationary phase and a decline phase. In the log phase the number doubles at a steady pace, . A recombinant protein comes from a gene that is expressed in a host of another species.
The worked solution
Have you tried it?
Work it out on paper first. It is fine to get stuck or make a mistake: that is how we learn. When you are ready, go through the solution one step at a time, and check each step against your own.
Given. Start . Target . Doubling time min.
Plan. The target is times the start. Find the smallest with .
Step 1. (a) is too small. is enough. So doublings.
Step 2. (b) Time: minutes.
Answer: doublings; minutes.
Check. After doublings the culture holds cells in every millilitre, which is above the target.
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