A company wants 200 g of a finished protein…
Contributed by StemPy LabTry it on paper first. A hint, the key idea and the worked solution are here when you need them.
A company wants g of a finished protein. Its downstream processing keeps per cent in the first step, per cent in the second step and per cent in the third step.
- What is the overall yield, as a per cent?
- How many grams of the protein must the culture hold at the end of the batch?
Need a reminder? The key ideaShow
Downstream processing is separation, purification, formulation with preservatives and quality control. A drug also goes through clinical trials. The overall yield is the product of the yields of the steps.
The worked solution
Have you tried it?
Work it out on paper first. It is fine to get stuck or make a mistake: that is how we learn. When you are ready, go through the solution one step at a time, and check each step against your own.
Given. Target g. Shares , and .
Plan. Overall yield the product of the shares. Starting amount target overall yield.
Step 1. (a) , which is per cent.
Step 2. (b) g.
Answer: per cent; g.
Check. g.
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