The Tool Kit of Genetic Engineering

Name: The Tool Kit of Genetic Engineering
Author: StemPy Lab

Two enzymes and one small circle of DNA are enough to move a gene from one organism to another. You learn what biotechnology is and what its two core techniques do. Then you meet the restriction enzymes that cut DNA at a palindrome, the ligases that join it again, and the vectors and hosts that carry and copy it. It serves the Indian syllabus (NCERT, NEET) and the matching topic of A-Level, IB and AP.

3 lessons, about 1 h 30 min3 simulations11 problems3 practice questionsPython in every lesson

Created by StemPy Lab

Course outline (3 lessons)

Lesson 1about 30 min
Biotechnology and its two core techniques
Biotechnology uses living cells or their parts to make useful products and services. It has two core techniques. Genetic engineering changes the genetic material and puts it into a host. Bioprocess engineering keeps the surroundings sterile so that only the wanted cells grow in large amounts. This lesson gives the meaning, the three basic steps of making a recombinant DNA, and the tool kit.
1 simulation1 practice question1 Python program3 problems
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Lesson 2about 30 min
Restriction enzymes: scissors that read a sequence
Restriction enzymes are cutting enzymes that come from bacteria. Each one recognises a short palindromic sequence of bases and cuts both strands inside it. Many of them leave sticky ends. This lesson explains the name of an enzyme, a palindrome, sticky and blunt ends, and the counting of fragments.
1 simulation1 practice question1 Python program5 problems1 Python lab
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Lesson 3about 30 min
Ligases, polymerases, vectors and hosts
DNA ligase joins pieces of DNA, and polymerases copy them. A vector carries the gene into a host and makes copies of it. A good vector has an origin of replication, a selectable marker and cloning sites. The plasmid pBR322 is the example in NCERT. The host must be made competent to take up the DNA.
1 simulation1 practice question1 Python program3 problems1 Python lab1 question set1 note
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The Tool Kit of Genetic Engineering: Timed Test

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The Tool Kit of Genetic Engineering

Course outline (3 lessons)

Biotechnology and its two core techniques

Restriction enzymes: scissors that read a sequence

Ligases, polymerases, vectors and hosts

Test Your Learning

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