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How was human insulin made by recombinant DNA… | StemPy Lab
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How was human insulin made by recombinant DNA technology in 1983?
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High School (11–12)
Biotechnology › Biotechnology and its Applications
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How was human insulin made by recombinant DNA technology in 1983?
Chains A and B were made separately in
E. coli
and joined by disulphide bonds
One bacterium made the whole pro-hormone with the C peptide in it
Chains A and B were cut out of the pancreas of cattle and joined
The C peptide was added to chains A and B in the laboratory
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