Use the model of the lesson: distance = (62 - 14 log₁₀ size) × time/60, in millimetres, for a run…
Contributed by StemPy LabTry it on paper first. A hint, the key idea and the worked solution are here when you need them.
Use the model of the lesson: distance , in millimetres, for a run of a given time in minutes. A digest gives fragments of bp, bp and bp. The gel runs for minutes.
- How far from the well does the bp band lie?
- How far does the bp band lie?
- How far does the bp band lie?
Need a reminder? The key ideaShow
The enzyme that opens a cell depends on its wall: lysozyme for bacteria, cellulase for plants, chitinase for fungi. Ribonuclease removes RNA, protease removes proteins, and chilled ethanol makes DNA appear. In a gel, DNA moves to the anode, and smaller fragments move farther. In the model of the lesson, distance .
The worked solution
Have you tried it?
Work it out on paper first. It is fine to get stuck or make a mistake: that is how we learn. When you are ready, go through the solution one step at a time, and check each step against your own.
Given. Sizes , and bp. Time min.
Plan. Use distance .
Step 1. (a) . Distance: mm.
Step 2. (b) . Distance: mm.
Step 3. (c) . Distance: mm.
Answer: mm; mm; mm.
Check. The smaller the fragment, the greater the distance. The order is right.
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