Try it on paper first. A hint, the key idea and the worked solution are here when you need them.
E. coli is grown for many generations in a medium with as the only source of nitrogen, so its DNA is heavy. The cells are then moved to a medium with and keep dividing, once every . Samples of DNA are separated by density in a caesium chloride gradient. Follow one heavy DNA molecule present at the time of the move.
Have you tried it?
Work it out on paper first. It is fine to get stuck or make a mistake: that is how we learn. When you are ready, go through the solution one step at a time, and check each step against your own.
Generations. at one division every is generations, so the one molecule becomes molecules.
(b) Hybrid and light. After the first generation both molecules are hybrid: each has one strand and one new strand. From then on, each heavy strand stays in one hybrid molecule, and all the other molecules have two light strands. So there are always hybrid molecules, and light ones.
(c) Percentage hybrid. , so hybrid : light .
(d) Back to . After ( generations) there are hybrid and light molecules. One more generation in pairs every old strand with a new heavy strand. Each hybrid molecule gives one heavy molecule ( with ) and one hybrid. Each light molecule gives two hybrids. That is heavy and hybrid out of : heavy and hybrid, with no light DNA.
Answer: (a) molecules. (b) hybrid and light. (c) . (d) heavy and hybrid.
Check. Count strands. After there are strands, and only the original are heavy. They sit in different molecules, so molecules are hybrid. In (d), of the strands are old strands, are old strands, and the new strands are all heavy.
Watch out. is not radioactive. The heavy and light DNA are told apart only by their densities in the caesium chloride gradient.
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