Try it on paper first. A hint, the key idea and the worked solution are here when you need them.
A ring of DNA has base pairs. Enzyme A cuts it at and . Enzyme B cuts it at and . The positions are measured from a fixed start.
In a gel, the size of a fragment falls in a straight line against the distance on a log scale. A ring cut at sites gives pieces, and a straight DNA gives . In PCR, copies are made from one molecule in cycles. The Wallace rule is in degrees Celsius.
Have you tried it?
Work it out on paper first. It is fine to get stuck or make a mistake: that is how we learn. When you are ready, go through the solution one step at a time, and check each step against your own.
Given. Ring of 9000. A cuts at 1500 and 5000. B cuts at 3000 and 7500.
Plan. Find the distances between the cuts, round the ring.
Step 1. (a) A ring cut twice gives 2 pieces: and .
Step 2. (b) For B: and . The two pieces are equal, so they run as one band.
Step 3. (c) Together the cuts are at 1500, 3000, 5000 and 7500. The pieces are , , , and . The largest is 3000.
Answer: ; ; bp.
Check. .
Log in to join the discussion.
Loading…